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Literature summary for 4.2.99.18 extracted from
- Saccharomyces cerevisiae possesses two functional homologues of Escherichia coli endonuclease III (1998), Biochemistry, 37, 6033-6040.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Scr1 and Scr2 | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00016 | - |
37mer with dihydrouridine | pH 6.8, 37°C, Scr2 | Saccharomyces cerevisiae | |
| 0.000227 | - |
37mer with dihydrouridine | pH 6.8, 37°C, Scr1 | Saccharomyces cerevisiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | Scr2 but not Scr1 is an iron-sulfur protein | Saccharomyces cerevisiae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | Saccharomyces cerevisiae | - |
fragments of DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Scr1 and Scr2 | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 37mer with dihydrouridine | - |
Saccharomyces cerevisiae | ? | - |
? | |
| DNA | - |
Saccharomyces cerevisiae | fragments of DNA | - |
? | |
| DNA | recognizes and cleaves DNA substrates containing dihydrouracil, 2,6-diamino-4-hydroxy-5N-methylformamidopyrimidine and abasic sites, but not DNA substrates containing uracil or 8-oxoguanine | Saccharomyces cerevisiae | fragments of DNA | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00833 | - |
37mer with dihydrouridine | pH 6.8, 37°C, Scr2 | Saccharomyces cerevisiae | |
| 0.0717 | - |
37mer with dihydrouridine | pH 6.8, 37°C, Scr1 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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