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Literature summary for 4.2.99.18 extracted from

  • Lebedeva, N.A.; Khodyreva, S.N.; Favre, A.; Lavrik, O.I.
    AP endonuclease 1 has no biologically significant 3'->5'-exonuclease activity (2002), Biochem. Biophys. Res. Commun., 300, 182-187.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
D283A altered kinetic values Homo sapiens
D283A/D308A altered kinetic values compared to wild-type enzyme Homo sapiens
D308A altered kinetic values compared to wild-type enzyme Homo sapiens
H309N altered kinetic values compared to wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00005
-
oligomer with G/U pair D308A mutant, pH 7.5 Homo sapiens
0.0001
-
oligomer with G/U pair wild-type, pH 7.5 Homo sapiens
0.00012
-
oligomer with G/U pair D283A mutant, pH 7.5 Homo sapiens
0.00024
-
oligomer with G/U pair D283/D308A mutant, pH 7.5 Homo sapiens
0.0013
-
oligomer with G/U pair H309N mutant, pH 7.5 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Homo sapiens
-
fragments of DNA
-
?
additional information Homo sapiens key enzyme in repair of oxidatively damaged DNA ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
Homo sapiens fragments of DNA
-
?
additional information enzyme has no biologically significant 3'-5'-exonuclease activity, the activity only manifests at enzyme concentrations elevated by 6-7 orders magnitude, activity does not show a preference to mismatched compared to matched DNA structures as well as to nicked or gapped DNA substrates Homo sapiens ?
-
?
additional information key enzyme in repair of oxidatively damaged DNA Homo sapiens ?
-
?
oligomer with G/U pair
-
Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
oligomer with G/U pair H309N mutant, pH 7.5 Homo sapiens
0.02
-
oligomer with G/U pair D283/D308A mutant, pH 7.5 Homo sapiens
0.3
-
oligomer with G/U pair D283A mutant, pH 7.5 Homo sapiens
1.2
-
oligomer with G/U pair D308A mutant, pH 7.5 Homo sapiens
10
-
oligomer with G/U pair wild-type, pH 7.5 Homo sapiens