Literature summary for 4.2.99.18 extracted from

Ikeda, S.; Biswas, T.; Roy, R.; Izumi, T.; Boldogh, I.; Kurosky, A.; Sarker, A.H.; Seki, S.; Mitra, S.
Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue (1998), J. Biol. Chem., 273, 21585-21593.

Search for more literature for 4.2.99.18

Cloned(Commentary)

Cloned (Comment)	Organism
fusion protein with glutathione S-transferase	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K212R	lower catalytic specificity than wild-type enzyme	Homo sapiens
additional information	protein with two additional amino acid residues and a truncated protein with deletion of 22 residues at the amino-terminus are equally active	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	50% inhibition at 5-10 mM	Homo sapiens
Mg2+	50% inhibition at 5-10 mM	Homo sapiens
Mn2+	50% inhibition at 5-10 mM	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000069	-	DNA containing dihydrouracil	wild-type, pH 8, 37°C	Homo sapiens
0.00017	-	DNA containing dihydrouracil	K212R mutant, pH 8, 37°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Homo sapiens	5737	-
nucleus	-	Homo sapiens	5634	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	iron-sulfur protein	Homo sapiens
Iron	[4Fe-4S]-cluster	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA	Homo sapiens	-	fragments of DNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P78549	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA	-	Homo sapiens	fragments of DNA	-	?
DNA	cleaves thymine glycol-containing form I plasmid DNA and a dihydrouracil-containing oligonucleotide duplex	Homo sapiens	fragments of DNA	-	?
DNA containing dihydrouracil	-	Homo sapiens	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0000567	-	DNA containing dihydrouracil	K212R mutant, pH 8, 37°C	Homo sapiens
0.002	-	DNA containing dihydrouracil	wild-type, pH 8, 37°C	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Homo sapiens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Homo sapiens	-	-	10

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Release 2023.1 (January 2023)