Any feedback?
Literature summary for 4.2.99.18 extracted from
- Isolation Isolation and characterization of a magnesium-deoendebt apurinic sites specific endodeoxyribonuclease from BAcillus subtilis (1980), Agric. Biol. Chem., 44, 567-574.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | complete inhibition at 1 mM | Bacillus subtilis |  |
| EDTA | - |
Bacillus subtilis | |
| Mg2+ | MgCl2 above 20 mM | Bacillus subtilis | |
| NaCl | 1 mM: complete inhibition | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Bacillus subtilis | |
| Mg2+ | optimal concentration: 20 mM | Bacillus subtilis | |
| additional information | Mn2+ has no effect | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
4 * 26000, SDS-PAGE | Bacillus subtilis |
| 105000 | - |
glycerol gradient centrifugation | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | Bacillus subtilis | - |
fragments of DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spore | germinating | Bacillus subtilis | - |
| vegetative | - |
Bacillus subtilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | - |
Bacillus subtilis | fragments of DNA | - |
? | |
| DNA | hydrolyzes phosphodiester bond near heat-induced apruinic sites in double or single strand DNA | Bacillus subtilis | fragments of DNA | - |
? | |
| DNA | specific for apurinic sites | Bacillus subtilis | fragments of DNA | - |
? | |
| additional information | no substrate: alkylated sites | Bacillus subtilis | ? | - |
? | |
| additional information | no substrate: native DNA | Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 26000, SDS-PAGE | Bacillus subtilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
