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Literature summary for 4.2.99.18 extracted from
- Purification and properties of endonuclease specific for apurinic sites of Bacillus stearothermophilus (1978), J. Biol. Chem., 253, 850-855.
General Stability
| General Stability | Organism |
|---|---|
| Escherichia coli enzyme is more sensitive to SDS, sodium deoxycholate or urea than the Bacillus stearothermophilus enzyme | Geobacillus stearothermophilus |
| stable up to 2% Triton X-100 | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 39% inhibition at 10 mM | Geobacillus stearothermophilus |  |
| Co2+ | 86% inhibition at 10 mM | Geobacillus stearothermophilus | |
| Cu2+ | 90% inhibition at 10 mM | Geobacillus stearothermophilus | |
| EDTA | no inhibition | Geobacillus stearothermophilus | |
| Mg2+ | 57% inhibition at 10 mM | Geobacillus stearothermophilus | |
| Mn2+ | 37% inhibition at 10 mM | Geobacillus stearothermophilus | |
| Zn2+ | 86% inhibition at 10 mM | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | stimulates | Geobacillus stearothermophilus | |
| KCl | optimal concentration: 125 mM | Geobacillus stearothermophilus | |
| NaCl | stimulates | Geobacillus stearothermophilus | |
| NaCl | optimal concentration: 50 mM | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration | Geobacillus stearothermophilus |
| 28000 | - |
1 * 28000, SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | Geobacillus stearothermophilus | - |
fragments of DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | - |
Geobacillus stearothermophilus | fragments of DNA | - |
? | |
| DNA | specific for apurinic sites | Geobacillus stearothermophilus | fragments of DNA | - |
? | |
| additional information | no substrate: alkylated sites | Geobacillus stearothermophilus | ? | - |
? | |
| additional information | no substrate: native DNA | Geobacillus stearothermophilus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
with 0.4% bovine serum albumin stable for 120 min | Geobacillus stearothermophilus |
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