Literature summary for 4.2.3.7 extracted from

Cane, D.E.; Sohng, J.K.; Lamberson, C.R.; Rudnicki, S.M.; Wu, Z.; Lloyd, M.D.; Oliver, J.S.; Hubbard, B.R.
Pentalenene synthase. Purification, molecular cloning, sequencing, and high-level expression in Escherichia coli of a terpenoid cyclase from Streptomyces UC5319 (1994), Biochemistry, 33, 5846-5857.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Streptomyces exfoliatus

Inhibitors

Inhibitors	Comment	Organism	Structure
diphosphate	-	Streptomyces exfoliatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00031	-	farnesyl diphosphate	30°C, pH 8.2	Streptomyces exfoliatus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	absolute requirement	Streptomyces exfoliatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41000	-	x * 41000, SDS-PAGE	Streptomyces exfoliatus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces exfoliatus	Q55012	UC5319	-
Streptomyces exfoliatus UC5319	Q55012	UC5319	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptomyces exfoliatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.324	-	30°C, pH 8.2	Streptomyces exfoliatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
farnesyl diphosphate	-	Streptomyces exfoliatus	pentalenene + diphosphate	-	?
farnesyl diphosphate	-	Streptomyces exfoliatus UC5319	pentalenene + diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 41000, SDS-PAGE	Streptomyces exfoliatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.2	8.4	-	Streptomyces exfoliatus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0032	-	diphosphate	-	Streptomyces exfoliatus

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Release 2023.1 (January 2023)