Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.6 extracted from

  • Vedula, L.S.; Jiang, J.; Zakharian, T.; Cane, D.E.; Christianson, D.W.
    Structural and mechanistic analysis of trichodiene synthase using site-directed mutagenesis: probing the catalytic function of tyrosine-295 and the asparagine-225/serine-229/glutamate-233-Mg2+B motif (2008), Arch. Biochem. Biophys., 469, 184-194.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Escherichia coli BL21DE3 cells Fusarium sporotrichioides

Crystallization (Commentary)

Crystallization (Comment) Organism
the structures of the mutants are solved at resolutions ranging from 2.1 to 2.7 A Fusarium sporotrichioides

Protein Variants

Protein Variants Comment Organism
N225D mutation in the fungal NSE motif, responsible for chelating Mg2+, mutant generates a different distribution of sesquiterpene products Fusarium sporotrichioides
N225D, S229T mutation in the fungal NSE motif, responsible for chelating Mg2+, inactive mutant Fusarium sporotrichioides
S229T mutation in the fungal NSE motif, responsible for chelating Mg2+, mutant generates a different distribution of sesquiterpene products Fusarium sporotrichioides
Y295F mutation in the fungal NSE motif, responsible for chelating Mg2+, mutant generates a different distribution of sesquiterpene products Fusarium sporotrichioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000069
-
farnesyl diphosphate mutant Y295F Fusarium sporotrichioides
0.000078
-
farnesyl diphosphate wild-type Fusarium sporotrichioides
0.000485
-
farnesyl diphosphate mutant N225D Fusarium sporotrichioides
0.006
-
farnesyl diphosphate mutant S229T Fusarium sporotrichioides

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Fusarium sporotrichioides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
-
Fusarium sporotrichioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans,trans-farnesyl diphosphate Fusarium sporotrichioides
-
trichodiene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Fusarium sporotrichioides P13513
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Fusarium sporotrichioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans,trans-farnesyl diphosphate
-
Fusarium sporotrichioides trichodiene + diphosphate
-
?

Subunits

Subunits Comment Organism
homodimer
-
Fusarium sporotrichioides

Synonyms

Synonyms Comment Organism
trichodiene synthase
-
Fusarium sporotrichioides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
activity assay Fusarium sporotrichioides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
farnesyl diphosphate mutant N225D Fusarium sporotrichioides
0.015
-
farnesyl diphosphate mutant S229T Fusarium sporotrichioides
0.111
-
farnesyl diphosphate mutant Y295F Fusarium sporotrichioides
0.138
-
farnesyl diphosphate wild-type Fusarium sporotrichioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
activity assay Fusarium sporotrichioides