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Literature summary for 4.2.3.6 extracted from

  • Cane, D.E.; Shim, J.H.; Xue, Q.; Fitzsimons, B.C.
    Trichodiene synthase. Identification of active site residues by site-directed mutagenesis (1995), Biochemistry, 34, 2480-2488.
    View publication on PubMed
Search for more literature for 4.2.3.6

Protein Variants

Protein Variants Comment Organism
C146A complete loss of activity Fusarium sporotrichioides
C190A significantly reduced enzymatic activity Fusarium sporotrichioides
additional information hybrid of enzyme from both organisms, site directed mutagenesis of hybrid Fusarium sambucinum
additional information hybrid of enzyme from both organisms, site directed mutagenesis of hybrid Fusarium sporotrichioides

Inhibitors

Inhibitors Comment Organism Structure
additional information identification of active site residues by site-directed mutagenesis Fusarium sporotrichioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate Fusarium sambucinum
-
 trichodiene + diphosphate
-
 ?
(E,E)-farnesyl diphosphate Fusarium sporotrichioides
-
 trichodiene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium sambucinum
-
-
-
Fusarium sporotrichioides
-
 recombinant enzyme from Escherichia coli BL21 DE3/pZW03
-
Fusarium sporotrichioides
-
 mutant C146A and C190A constructed by site-directed mutagenesis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate
-
 Fusarium sambucinum trichodiene + diphosphate
-
 ?
(E,E)-farnesyl diphosphate
-
 Fusarium sporotrichioides trichodiene + diphosphate
-
 ?