Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces avermitilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000125 | - |
(2E,6E)-farnesyl diphosphate | pH 7.0, 30°C | Streptomyces avermitilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptomyces avermitilis | |
Mn2+ | 32% of the activity with Mg2+ | Streptomyces avermitilis | |
Zn2+ | 5% of the activity with Mg2+ | Streptomyces avermitilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | Q82IV1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Streptomyces avermitilis | (+)-epi-isozizaene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SAV_3032 | - |
Streptomyces avermitilis |
TPS2 | - |
Streptomyces avermitilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces avermitilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00026 | - |
(2E,6E)-farnesyl diphosphate | pH 7.0, 30°C | Streptomyces avermitilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptomyces avermitilis |
General Information | Comment | Organism |
---|---|---|
physiological function | Streptomyces avermitilis does not produce epi-isozizaene or any of its oxidized derivatives, albaflavenols and albaflavenone, in any culture conditions examined. Recombinant SAV_3032 protein expressed in Escherichia coli catalyses the Mg2+-dependent cyclization of farnesyl diphosphate to epi-isozizaene | Streptomyces avermitilis |