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Literature summary for 4.2.3.37 extracted from

  • Aaron, J.A.; Lin, X.; Cane, D.E.; Christianson, D.W.
    Structure of epi-isozizaene synthase from Streptomyces coelicolor A3(2), a platform for new terpenoid cyclization templates (2010), Biochemistry, 49, 1787-1797.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.37

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli BL21(DE3) Streptomyces coelicolor

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
D99N coordinates to Mg2+ Streptomyces coelicolor
F198A major products: Z-gamma-bisabolene (24%), sesqusabine (20%) Streptomyces coelicolor
F96A major product: beta-farnesene (70%) Streptomyces coelicolor
W203F major product: Z-gamma-bisabolene (47%) Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00071
-
 (2E,6E)-farnesyl diphosphate wild-type protein Streptomyces coelicolor
0.00077
-
 (2E,6E)-farnesyl diphosphate F96A mutant protein Streptomyces coelicolor
0.0012
-
 (2E,6E)-farnesyl diphosphate F198A mutant protein Streptomyces coelicolor
0.00145
-
 (2E,6E)-farnesyl diphosphate F203F mutant protein Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9K499
-
-
Streptomyces coelicolor A3(2) Q9K499
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Co2+) Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Streptomyces coelicolor (+)-epi-isozizaene + diphosphate major product (79%) ?
(2E,6E)-farnesyl diphosphate
-
 Streptomyces coelicolor A3(2) (+)-epi-isozizaene + diphosphate major product (79%) ?

Synonyms

Synonyms Comment Organism
epi-isozizaene synthase
-
 Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00024
-
 (2E,6E)-farnesyl diphosphate F96A mutant protein Streptomyces coelicolor
0.0003
-
 (2E,6E)-farnesyl diphosphate F198A mutant protein Streptomyces coelicolor
0.00034
-
 (2E,6E)-farnesyl diphosphate F203F mutant protein Streptomyces coelicolor
0.045
-
 (2E,6E)-farnesyl diphosphate wild-type protein Streptomyces coelicolor

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.23
-
 (2E,6E)-farnesyl diphosphate F203F mutant protein Streptomyces coelicolor
0.25
-
 (2E,6E)-farnesyl diphosphate F198A mutant protein Streptomyces coelicolor
0.31
-
 (2E,6E)-farnesyl diphosphate F96A mutant protein Streptomyces coelicolor
63
-
 (2E,6E)-farnesyl diphosphate wild-type protein Streptomyces coelicolor