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Literature summary for 4.2.3.16 extracted from

  • Colby, S.M.; Alonso, W.R.; Katahira, E.J.; McGarvey, D.J.; Croteau, R.
    4S-limonene synthase from the oil glands of spearmint (Mentha spicata): cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase (1993), J. Biol. Chem., 268, 23016-23024.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mentha sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ cofactor Mentha sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 SDS-PAGE, gel filtration Mentha sp.
59800
-
 calculated from cDNA sequence Mentha sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate Mentha sp.
-
 (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and myrcene as side products ?

Organism

Organism UniProt Comment Textmining
Mentha sp.
-
 M. spicata
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mentha sp.

Source Tissue

Source Tissue Comment Organism Textmining
leaf glandular trichome secretory cells Mentha sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate
-
 Mentha sp. (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and myrcene as side products ?