Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant Cop3 in Escherichia coli strain JM109 | Coprinopsis cinerea |
Protein Variants | Comment | Organism |
---|---|---|
H255P | site-directed mutagenesis, modification of the H-alpha1 loop, the mutant shows highly altered product profile compared to the wild-type enzyme, it does no longer produce alpha-muurolene but large amounts of germacrene A (77%), overview | Coprinopsis cinerea |
K251I | site-directed mutagenesis, modification of the H-alpha1 loop, the mutant shows only slightly altered product profile compared to the wild-type enzyme, it produces also beta-copaene, cf. EC 4.2.3.127, overview | Coprinopsis cinerea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | Coprinopsis cinerea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Coprinopsis cinerea | |
additional information | the enzyme contains the metal-binding DDXXD motif | Coprinopsis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Coprinopsis cinerea | the major cyclization products of Cop3 and its loop mutants are derived from an E,E-germacradienyl cation formed after ionization and 1,10 cyclization of E,E-farnesyl diphosphate. alpha-Muurolene is 30% of the products formed from (2E,6E)-farnesyl diphosphate | alpha-muurolene + diphosphate | - |
? | |
additional information | Coprinopsis cinerea | seven different sesquiterpenes products, wild-type Cop3 also shows gamma-muurolene synthase activity, EC 4.2.3.126, and produces 12% gamma-muurolene and 35% gemacrene A, product of germacrene A synthase, EC 4.2.3.23, of total products from (2E,6E)-farnesyl diphosphate, product profiling by GC-MS analysis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Coprinopsis cinerea | alpha-muurolene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | the major cyclization products of Cop3 and its loop mutants are derived from an E,E-germacradienyl cation formed after ionization and 1,10 cyclization of E,E-farnesyl diphosphate. alpha-Muurolene is 30% of the products formed from (2E,6E)-farnesyl diphosphate | Coprinopsis cinerea | alpha-muurolene + diphosphate | - |
? | |
additional information | seven different sesquiterpenes products, wild-type Cop3 also shows gamma-muurolene synthase activity, EC 4.2.3.126, and produces 12% gamma-muurolene and 35% gemacrene A, product of germacrene A synthase, EC 4.2.3.23, of total products from (2E,6E)-farnesyl diphosphate, product profiling by GC-MS analysis, overview | Coprinopsis cinerea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structural homology modeling of Cop3 using the crystal structure of aristolochene synthase from Aspergillus terreus. Several polar side chains in the H-alpha1 loops of Cop4 and Cop3 move closer to side chains in the metal-binding DDXXD motif | Coprinopsis cinerea |
Synonyms | Comment | Organism |
---|---|---|
Cop3 | - |
Coprinopsis cinerea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Coprinopsis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Coprinopsis cinerea |
General Information | Comment | Organism |
---|---|---|
additional information | role of the active site H-alpha1 loop in catalysis, overview | Coprinopsis cinerea |