Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosylmethionine | allosteric activator, 4fold increase of Vmax, 3.3fold decrease of substrate Km at 0.4 mM | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | 80% inhibition at 1 mM in presence of S-adenosylmethionine | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | 2.7 | O-phosphohomoserine | - |
Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
190000 | - |
gel filtration | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
- |
Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phospho-L-homoserine + H2O | - |
Pisum sativum | L-threonine + phosphate | - |
ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Pisum sativum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.4 | - |
Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | maximal activity at 0.4-1.0 mM | Pisum sativum | |
pyridoxal 5'-phosphate | required cofactor | Pisum sativum |