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Literature summary for 4.2.2.16 extracted from

  • Cha, J.; Park, N.H.; Yang, S.J.; Lee, T.H.
    Molecular and enzymatic characterization of a levan fructotransferase from Microbacterium sp. AL-210 (2001), J. Biotechnol., 91, 49-61.
    View publication on PubMed
Search for more literature for 4.2.2.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Microbacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 10 mM, 81% inhibition Microbacterium sp.
AgNO3 10 mM, 62% inhibition Microbacterium sp.
EDTA 10 mM, 17% inhibition Microbacterium sp.
FeCl2 10 mM, 61% inhibition Microbacterium sp.
KMnO4 10 mM, 52% inhibition Microbacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 10 mM, activation to 127% of control Microbacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 gel filtration Microbacterium sp.

Organism

Organism UniProt Comment Textmining
Microbacterium sp.
-
-
-
Microbacterium sp. AL-210
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Microbacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Microbacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
levan levan from Zymomonas mobilis: conversion yield 28.4% after 24 h. Levan from Serratia: conversion yield: 28.4% after 24 h Microbacterium sp. 5 di-D-fructose-2,6':2',6 dianhydride + ?
-
 ?
levan levan from Zymomonas mobilis: conversion yield 28.4% after 24 h. Levan from Serratia: conversion yield: 28.4% after 24 h Microbacterium sp. AL-210 5 di-D-fructose-2,6':2',6 dianhydride + ?
-
 ?
additional information no activity with dextran, soluble starch, inulin, maltose, sucrose, raffinose, 1-kestose, nystose and 1F-fructofuranosyl nystose Microbacterium sp. ?
-
 ?
additional information no activity with dextran, soluble starch, inulin, maltose, sucrose, raffinose, 1-kestose, nystose and 1F-fructofuranosyl nystose Microbacterium sp. AL-210 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 46000, SDS-PAGE Microbacterium sp.

Synonyms

Synonyms Comment Organism
levan fructotransferase
-
 Microbacterium sp.
LFTase
-
 Microbacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Microbacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Microbacterium sp.
50
-
 activity diminishes quickly above Microbacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Microbacterium sp.