Literature summary for 4.2.2.15 extracted from

Gut, H.; King, S.J.; Walsh, M.A.
Structural and functional studies of Streptococcus pneumoniae neuraminidase B: An intramolecular trans-sialidase (2008), FEBS Lett., 582, 3348-3352.

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Cloned(Commentary)

Cloned (Comment)	Organism
NanB sequence lacking the first 29 residues is PCR-amplified from Streptococcus pneumoniae DNA, cloned into the pOPINF vector and expressed in Escherichia coli	Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of NanB in complex with its reaction product 2,7-anhydro-Neu5Ac is reported. NanB crystal structure consists of an N-terminal lectin-like domain (L-domain, residues 30-227), a catalytic six-bladed beta-propeller domain (N-domain, residues 228-345 and 457-697), and an irregular beta-stranded domain inserted into the catalytic domain (I-domain, residues 346-456)	Streptococcus pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
human alpha-1 acid glycoprotein + H2O	NanB preferentially cleaves alpha2-3 linked sialic acid from human alpha-1 glycoprotein	Streptococcus pneumoniae	2,7-anhydro-alpha-N-acetylneuraminic acid + ?	-	?

Synonyms

Synonyms	Comment	Organism
NanB	-	Streptococcus pneumoniae
neuraminidase B	-	Streptococcus pneumoniae

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Release 2023.1 (January 2023)