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Literature summary for 4.2.2.15 extracted from
- Purification and characterization of sialidase L, a NeuAc alpha(2-3)Gal-specific sialidase (1994), J. Biol. Chem., 269, 18821-18826.
General Stability
| General Stability | Organism |
|---|---|
| 50% loss of activity after one freeze and thawing cycle, in presence of 10% glycerol stable to several freeze and thawing cycles | Macrobdella decora |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not: 2-deoxy-2,3-dehydroacetylneuraminic acid, EDTA, Ca2+, Mg2+, Mn2+ | Macrobdella decora |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.68 | - |
4-methylumbelliferyl-alpha-N-acetylneuraminic acid | pH 5.5, 37°C | Macrobdella decora |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
x * 84000, SDS-PAGE | Macrobdella decora |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-N-acetylneuraminic acid glycoproteins or ganglioside + H2O | Macrobdella decora | - |
2,7-anhydro-alpha-N-acetylneuraminic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Macrobdella decora | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Macrobdella decora |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Macrobdella decora |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10% glycerol, stable for more than 4 months | Macrobdella decora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-N-acetylneuraminic acid + H2O | strict specificity towards the hydrolysis of NeuAcalpha(2-3)Gal-linkages | Macrobdella decora | 4-methylumbelliferone + 2,7-anhydro-alpha-N-acetylneuraminic acid | - |
? | |
| 4-methylumbelliferyl-alpha-N-acetylneuraminic acid glycoproteins or ganglioside + H2O | - |
Macrobdella decora | 2,7-anhydro-alpha-N-acetylneuraminic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 84000, SDS-PAGE | Macrobdella decora |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Macrobdella decora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | - |
Macrobdella decora |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Macrobdella decora | isoelectric focusing | - |
6 |
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Release 2023.1 (January 2023)
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