Cloned (Comment) | Organism |
---|---|
gene HEM4, library screening and complementation studies, sequence comparisons, expression of the enzyme in the knockout Saccharomyces cerevisiae S150-2B strain, overvexpression of the His-tagged enzyme in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | exclusive localization in chloroplasts, UROS has an N-terminal extension acting as a targeting sequence for import of the precursor protein of 34 kD into chloroplasts, followed by processing to the mature size of 29 kDa | Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 34000, precursor enzyme, x * 29000, mature enzyme | Arabidopsis thaliana |
34000 | - |
x * 34000, precursor enzyme, x * 29000, mature enzyme | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxymethylbilane | Arabidopsis thaliana | a step in tetrapyrrole biosynthesis, e.g. of chlorophyll, haem, sirohaem and bilins, overview | uroporphyrinogen III + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O48721 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the N-terminal extension of UROS is processed resulting in the mature enzyme of 29 kDa | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration to homogeneity | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxymethylbilane | a step in tetrapyrrole biosynthesis, e.g. of chlorophyll, haem, sirohaem and bilins, overview | Arabidopsis thaliana | uroporphyrinogen III + H2O | - |
? | |
hydroxymethylbilane | uroporphyrinogen III synthase catalyses the cyclization of HMB with a concomitant inversion of the fourth ring of the porphyrin macrocycle, giving rise to uroporphyrinogen III | Arabidopsis thaliana | uroporphyrinogen III + H2O | - |
? | |
additional information | coupled assay method for UROS activity measurement using purified recombinant Pseudomonas aeruginosa porphobilinogen synthase and Bacillus megaterium porphobilinogen deaminase to generate hydroxymethylbilane, the substrate for UROS, enzymatically from 5-aminolaevulinic acid | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, precursor enzyme, x * 29000, mature enzyme | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
Uroporphyrinogen III synthase | - |
Arabidopsis thaliana |
UROS | - |
Arabidopsis thaliana |