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Literature summary for 4.2.1.75 extracted from

  • Tan, F.; Cheng, Q.; Saha, K.; Heinemann, I.U.; Jahn, M.; Jahn, D.; Smith, A.G.
    Identification and characterization of the Arabidopsis gene encoding the tetrapyrrole biosynthesis enzyme uroporphyrinogen III synthase (2008), Biochem. J., 410, 291-299.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene HEM4, library screening and complementation studies, sequence comparisons, expression of the enzyme in the knockout Saccharomyces cerevisiae S150-2B strain, overvexpression of the His-tagged enzyme in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast exclusive localization in chloroplasts, UROS has an N-terminal extension acting as a targeting sequence for import of the precursor protein of 34 kD into chloroplasts, followed by processing to the mature size of 29 kDa Arabidopsis thaliana 9507
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
x * 34000, precursor enzyme, x * 29000, mature enzyme Arabidopsis thaliana
34000
-
x * 34000, precursor enzyme, x * 29000, mature enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxymethylbilane Arabidopsis thaliana a step in tetrapyrrole biosynthesis, e.g. of chlorophyll, haem, sirohaem and bilins, overview uroporphyrinogen III + H2O
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O48721
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the N-terminal extension of UROS is processed resulting in the mature enzyme of 29 kDa Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration to homogeneity Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxymethylbilane a step in tetrapyrrole biosynthesis, e.g. of chlorophyll, haem, sirohaem and bilins, overview Arabidopsis thaliana uroporphyrinogen III + H2O
-
?
hydroxymethylbilane uroporphyrinogen III synthase catalyses the cyclization of HMB with a concomitant inversion of the fourth ring of the porphyrin macrocycle, giving rise to uroporphyrinogen III Arabidopsis thaliana uroporphyrinogen III + H2O
-
?
additional information coupled assay method for UROS activity measurement using purified recombinant Pseudomonas aeruginosa porphobilinogen synthase and Bacillus megaterium porphobilinogen deaminase to generate hydroxymethylbilane, the substrate for UROS, enzymatically from 5-aminolaevulinic acid Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 34000, precursor enzyme, x * 29000, mature enzyme Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
Uroporphyrinogen III synthase
-
Arabidopsis thaliana
UROS
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Arabidopsis thaliana