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Literature summary for 4.2.1.75 extracted from

  • Li, N.; Ma, D.L.; Liu, X.; Wu, L.; Chu, X.; Wong, K.Y.; Li, D.
    Characterization of His-tagged rat uroporphyrinogen III synthase wild-type and variant enzymes (2007), Protein J., 26, 569-576.
    View publication on PubMed
Search for more literature for 4.2.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D17A 60.3% of wild-type activity Rattus norvegicus
E68A 82.8% of wild-type activity Rattus norvegicus
H173L 106.9% of wild-type activity Rattus norvegicus
K10Q 39.7% of wild-type activity Rattus norvegicus
K147Q 34.5% of wild-type activity Rattus norvegicus
N121D 93.1% of wild-type activity Rattus norvegicus
Y168F 1.7% of wild-type activity Rattus norvegicus
Y97F 74.1% of wild-type activity Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimal concentration is 100 mM, wild-type enzyme Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.7
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, stable for at least one year Rattus norvegicus
4 °C, stable for one week without significant change of activity Rattus norvegicus

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in both phosphate and Tris buffer Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 pH 7.5: about 60% of maximal activity, pH 9.0: about 70% of maximal activity Rattus norvegicus