Literature summary for 4.2.1.75 extracted from

Omata, Y.; Sakamoto, H.; Higashimoto, Y.; Hayashi, S.; Noguchi, M.
Purification and characterization of human uroporphyrinogen III synthase expressed in Escherichia coli (2004), J. Biochem., 136, 211-220.

Search for more literature for 4.2.1.75

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C73A	99% of wild-type activity	Homo sapiens
C73R	9% of wild-type activity	Homo sapiens
C73S	78% of wild-type activity	Homo sapiens

General Stability

General Stability	Organism
activity does not change after several cycles of freeze-and thawing in potassium phosphate buffer, pH 7.4, containing DTT and glycerol	Homo sapiens
recombinant enzyme in cell lysate is unstable, glycerol stabilizes	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.01	-	Hydroxymethylbilane	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28807	-	x * 28807, MALDI-TOF mass spectrometry	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P10746	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
54.7	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hydroxymethylbilane	-	Homo sapiens	uroporphyrinogen III + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 28807, MALDI-TOF mass spectrometry	Homo sapiens

Synonyms

Synonyms	Comment	Organism
uro'gen III synthase	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	purified enzyme shows remarkable thermostability, particularly when kept in phosphate buffer containing DTT and EDTA, indicating that the activity may depend on its oxidation state	Homo sapiens
37	-	about 20% loss of activity after 48 h, about 50% loss of activity after 72 h, buffer containing 1 mM DTT	Homo sapiens
45	-	about 10% loss of activity after 24 h, buffer containing 1 mM DTT	Homo sapiens
60	-	about 75% loss of activity after 3 min, buffer containing 1 mM DTT	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
30	-	Hydroxymethylbilane	-	Homo sapiens

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Release 2023.1 (January 2023)