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Literature summary for 4.2.1.75 extracted from

  • Stamford, N.P.J.; Capretta, A.; Battersby, A.R.
    Expression, purification and characterisation of the product from the Bacillus subtilis hemD gene, uroporphyrinogen III synthase (1995), Eur. J. Biochem., 231, 236-241.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Butane-2,3-dione
-
Bacillus subtilis
diethyl dicarbonate
-
Bacillus subtilis
NaN3 60% residual activity with 5.0 mM Bacillus subtilis
Phenylglyoxal
-
Bacillus subtilis
pyridoxal 5'-phosphate Pyridoxal 5'-phosphate and subsequent treatment with NaBH4 Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00033
-
Hydroxymethylbilane
-
Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information bivalent ions, such as MgCl2 up to 20 mM, fail to stimulate enzyme activity Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29100
-
nucleotide sequence Bacillus subtilis
32000
-
SDS-PAGE Bacillus subtilis
41500
-
gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Oxidation Stability

Oxidation Stability Organism
photooxidation in presence of Rose Bengal Bacillus subtilis

Purification (Commentary)

Purification (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.4
-
hydroxymethylbilane Bacillus subtilis

Storage Stability

Storage Stability Organism
-70¦C, at least two years Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hydroxymethylbilane
-
Bacillus subtilis Uroporphyrinogen III
-
?

Subunits

Subunits Comment Organism
monomer SDS-PAGE, gel filtration, nucleotide sequence Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
more thermostable than enzymes from other sources Bacillus subtilis
30
-
retention of 100% activity after 24 h Bacillus subtilis
45
-
retention of 90% activity after 24 h Bacillus subtilis
60
-
completely inactivated in 1 min Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6
-
Hydroxymethylbilane
-
Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Bacillus subtilis