Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Butane-2,3-dione | - |
Bacillus subtilis | |
diethyl dicarbonate | - |
Bacillus subtilis | |
NaN3 | 60% residual activity with 5.0 mM | Bacillus subtilis | |
Phenylglyoxal | - |
Bacillus subtilis | |
pyridoxal 5'-phosphate | Pyridoxal 5'-phosphate and subsequent treatment with NaBH4 | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
Hydroxymethylbilane | - |
Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | bivalent ions, such as MgCl2 up to 20 mM, fail to stimulate enzyme activity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29100 | - |
nucleotide sequence | Bacillus subtilis |
32000 | - |
SDS-PAGE | Bacillus subtilis |
41500 | - |
gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Oxidation Stability | Organism |
---|---|
photooxidation in presence of Rose Bengal | Bacillus subtilis |
Purification (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.4 | - |
hydroxymethylbilane | Bacillus subtilis |
Storage Stability | Organism |
---|---|
-70¦C, at least two years | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hydroxymethylbilane | - |
Bacillus subtilis | Uroporphyrinogen III | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE, gel filtration, nucleotide sequence | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
more thermostable than enzymes from other sources | Bacillus subtilis |
30 | - |
retention of 100% activity after 24 h | Bacillus subtilis |
45 | - |
retention of 90% activity after 24 h | Bacillus subtilis |
60 | - |
completely inactivated in 1 min | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
Hydroxymethylbilane | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Bacillus subtilis |