Literature summary for 4.2.1.59 extracted from

Nguyen, C.; Haushalter, R.; Lee, D.; Markwick, P.; Bruegger, J.; Caldara-Festin, G.; Finzel, K.; Jackson, D.; Ishikawa, F.; O'Dowd, B.; McCammon, J.; Opella, S.; Tsai, S.; Burkart, M.
Trapping the dynamic acyl carrier protein in fatty acid biosynthesis (2014), Nature, 505, 427-431.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 10 mM sodium phosphate (pH 8.0), 350 mM sodium acetate, 1 M LiCl and 35% (w/v) PEG3350	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(3R)-3-hydroxydecanoyl-[acyl-carrier protein]	Escherichia coli	-	2-decenoyl-[acyl-carrier protein] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A6Q3	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-affinity column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3R)-3-hydroxydecanoyl-[acyl-carrier protein]	-	Escherichia coli	2-decenoyl-[acyl-carrier protein] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
3-hydroxyacyl-ACP dehydratase	-	Escherichia coli

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Release 2023.1 (January 2023)