Any feedback?
Literature summary for 4.2.1.3 extracted from
- Biochemical characterisation of aconitase from Corynebacterium glutamicum (2011), J. Biotechnol., 154, 163-170.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of N-terminally His-tagged aconitase in Escherichia coli strain BL21(DE3), alternative expression method uses overexpression of aconitase from plasmid pTYB2-acn, resulting in an aconitase containing only an additional glycine residue at the C-terminus | Corynebacterium glutamicum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Corynebacterium glutamicum | |
| 0.0185 | - |
cis-aconitate | pH 8.0, 30°C | Corynebacterium glutamicum |  |
| 0.48 | - |
citrate | pH 8.0, 30°C | Corynebacterium glutamicum | |
| 0.552 | - |
isocitrate | pH 8.0, 30°C | Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 102300 | - |
1 * 104800, recombinant N-terminally His-tagged aconitase, sequence calculation, 1 * 102300, recombinant aconitase with an additional Gly at the C-terminus, sequence calculation | Corynebacterium glutamicum |
| 114000 | - |
recombinant N-terminally His-tagged aconitase, gel filtration | Corynebacterium glutamicum |
| 122000 | - |
recombinant aconitase with an additional Gly at the C-terminus, gel filtration | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrate | Corynebacterium glutamicum | - |
cis-aconitate + H2O | - |
r | |
| citrate | Corynebacterium glutamicum ATCC 13032 | - |
cis-aconitate + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
- |
- |
| Corynebacterium glutamicum ATCC 13032 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged aconitase from Escherichia coli strain BL21(DE3) by nickel affinty chromatography and gel filtration, removal of the His tag by factor Xa and further purification, or purification of recombinant aconitase, expressed from plasmid pTYB2-acn in Escherichia coli, using chitin beads, overview | Corynebacterium glutamicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:glucose-grown cell | - |
Corynebacterium glutamicum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.206 | - |
dehydration of citrate to cis-aconitate, pH 8.0, 30°C | Corynebacterium glutamicum |
| 0.266 | - |
dehydration of isocitrate to cis-aconitate, pH 8.0, 30°C | Corynebacterium glutamicum |
| 0.433 | - |
reverse reaction, hydration of citrate or isocitrate, pH 8.0, 30°C | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrate | - |
Corynebacterium glutamicum | cis-aconitate + H2O | - |
r | |
| citrate | - |
Corynebacterium glutamicum ATCC 13032 | cis-aconitate + H2O | - |
r | |
| isocitrate | - |
Corynebacterium glutamicum | cis-aconitate + H2O | - |
r | |
| isocitrate | - |
Corynebacterium glutamicum ATCC 13032 | cis-aconitate + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 104800, recombinant N-terminally His-tagged aconitase, sequence calculation, 1 * 102300, recombinant aconitase with an additional Gly at the C-terminus, sequence calculation | Corynebacterium glutamicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aconitase | - |
Corynebacterium glutamicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Corynebacterium glutamicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | - |
Corynebacterium glutamicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 7.75 | - |
Corynebacterium glutamicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | - |
Corynebacterium glutamicum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
