Cloned (Comment) | Organism |
---|---|
gene AcnA, overexpression of the His-tagged protein in an ASKA library, i.e. the 'a complete set of Escherichia coli K-12 ORF archive library | Escherichia coli |
gene ACnB, overexpression of the His-tagged protein in an ASKA library | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the iron-sulfur cluster, which constitutes the active site, is located at the interdomain boundary among the three enzyme domains, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-aconitate + H2O | Escherichia coli | aconitase catalyzes a reversible isomerization of citrate into isocitrate in the Krebs cycle | isocitrate | - |
r | |
citrate | Escherichia coli | aconitase catalyzes a reversible isomerization of citrate into isocitrate in the Krebs cycle | cis-aconitate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P25516 | AcnA; gene AcnA | - |
Escherichia coli | P36683 | AcnB; gene AcnB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged AcnA by metal affinity chromatography | Escherichia coli |
recombinant His-tagged AcnB by metal affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-aconitate + H2O | - |
Escherichia coli | isocitrate | - |
r | |
cis-aconitate + H2O | aconitase catalyzes a reversible isomerization of citrate into isocitrate in the Krebs cycle | Escherichia coli | isocitrate | - |
r | |
citrate | - |
Escherichia coli | cis-aconitate + H2O | - |
r | |
citrate | aconitase catalyzes a reversible isomerization of citrate into isocitrate in the Krebs cycle | Escherichia coli | cis-aconitate + H2O | - |
r | |
additional information | the substrate binding may induce a rearrangement of their relative positions. Such a conformational change may result in the negative cooperativity | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | structural modeling, homo-oligomerization of AcnA yields negative cooperativities in isomerization of isocitrate. In the AcnA homodimer, the intersubunit interface is composed of domains II and III. The iron-sulfur cluster, which constitutes the active site, is located at the interdomain boundary among the three domains, overview | Escherichia coli |
homodimer | structural modeling, homo-oligomerization of AcnB yields negative cooperativities in isomerization of isocitrate. In the AcnA homodimer, the intersubunit interface is composed of domains II and III. The iron-sulfur cluster, which constitutes the active site, is located at the interdomain boundary among the three domains, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
aconitase | - |
Escherichia coli |