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Literature summary for 4.2.1.3 extracted from

  • Banerjee, S.; Nandyala, A.K.; Raviprasad, P.; Ahmed, N.; Hasnain, S.E.
    Iron-dependent RNA-binding activity of Mycobacterium tuberculosis aconitase (2007), J. Bacteriol., 189, 4046-4052.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 isocitrate pH 8.0, 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Mycobacterium tuberculosis
102000
-
 1 * 102000, SDS-PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
 bifunctional enzyme with aconitase activity and working as iron-responsive protein. Since iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
118
-
 pH 8.0, 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
 Mycobacterium tuberculosis cis-aconitate + H2O
-
 ?
additional information in addition to aconitase activity, enzyme binds with high specificity to iron-responsive element-like RNA sequences. Iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive Mycobacterium tuberculosis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 102000, SDS-PAGE Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
 Mycobacterium tuberculosis