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Literature summary for 4.2.1.3 extracted from
- Studies on two isozymes of aconitase from Bacillus cereus T. III. Enzymatic properties (1976), Biochem. Biophys. Res. Commun., 70, 987-996.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethyl picolinate | isoenzyme is inhibited, isoenzyme I is less or not sensitive | Bacillus cereus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
isocitrate | isoenzyme II | Bacillus cereus | |
| 1.4 | - |
citrate | isoenzyme II | Bacillus cereus | |
| 3.33 | - |
isocitrate | isoenzyme I | Bacillus cereus | |
| 10 | - |
citrate | isoenzyme I | Bacillus cereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | 80000 | isoenzyme II, gel filtration | Bacillus cereus |
| 150000 | 160000 | isoenzyme I, gel filtration | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
early aconitase (isoenzyme I) is present in a culture of 5 h age, late aconitase (isoenzyme II) is present in 12 h age | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | isoenzyme I | Bacillus cereus |
| 30 | - |
isoenzyme II | Bacillus cereus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | about 60% of maximal activity at 20°C and at 40°C, isoenzyme I. 20°C: about 40% of maximal activity, 40°C: about 20% of maximal activity, isoenzyme II | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
isoenzyme II | Bacillus cereus |
| 7.5 | 8 | isoenzyme I | Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6: about 55% of maximal activity, pH 9: about 40% of maximal activity, isoenzyme I. pH 6: about 25% of maximal activity, pH 9: about 75% of maximal activity isoenzyme II | Bacillus cereus |
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