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Literature summary for 4.2.1.24 extracted from
- delta-Aminolevulinic acid dehydratase of Rhodopseudomonas capsulata (1973), Arch. Biochem. Biophys., 158, 305-311.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | required at very low concentrations of substrate | Rhodobacter capsulatus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | no inhibition | Rhodobacter capsulatus | |
| iodoacetamide | - |
Rhodobacter capsulatus | |
| additional information | insensitive to inhibition by hemin and protoporphyrin | Rhodobacter capsulatus | |
| NaCN | in presence of the substrate | Rhodobacter capsulatus | |
| NEM | - |
Rhodobacter capsulatus | |
| PCMB | - |
Rhodobacter capsulatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
5-aminolevulinate | in presence of 2-mercaptoethanol | Rhodobacter capsulatus | |
| 2 | - |
5-aminolevulinate | in absence of 2-mercaptoethanol | Rhodobacter capsulatus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | does not require metallic cations for activation | Rhodobacter capsulatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 260000 | - |
sucrose density gradient centrifugation | Rhodobacter capsulatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodobacter capsulatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodobacter capsulatus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 5-aminolevulinate = porphobilinogen + 2 H2O | substrate is covalently bound through a Schiff base | Rhodobacter capsulatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.62 | - |
- |
Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-aminolevulinate + 5-aminolevulinate | - |
Rhodobacter capsulatus | porphobilinogen + 2 H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.6 | in citrate buffer, Tris-maleate buffer, imidazole buffer and Tris buffer | Rhodobacter capsulatus |
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Release 2023.1 (January 2023)
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