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Literature summary for 4.2.1.20 extracted from

  • Evran, S.; Telefoncu, A.; Sterner, R.
    Directed evolution of (??)(8)-barrel enzymes: establishing phosphoribosylanthranilate isomerisation activity on the scaffold of the tryptophan synthase ?-subunit. (2012), Protein Eng., 25, 285-293.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli T7-express cells Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
D13N/F22C/T24A/K35E/D46N/D56N/P62S/E90K/G98C/L177D/Q210L/A222T the mutant shows phosphoribosylanthranilate isomerase activity Salmonella enterica subsp. enterica serovar Typhimurium
D13N/F22C/T24A/K35E/D56N/P62S/E90K/G98C/L144I/L177D/Q210L/I240V the mutant shows phosphoribosylanthranilate isomerase activity Salmonella enterica subsp. enterica serovar Typhimurium
D13N/F22C/T24A/K35E/D56N/P62S/G98C/L177D/Q210L the mutant shows phosphoribosylanthranilate isomerase activity Salmonella enterica subsp. enterica serovar Typhimurium
D13N/F22C/T24A/T39A/D60N/G61S/P62S/N66S/E90K/G98C/L177D/Q210L/A222T/I240V the mutant shows phosphoribosylanthranilate isomerase activity Salmonella enterica subsp. enterica serovar Typhimurium
F22C/T24A/K35E/D56N/P62S/E90K/G98C the mutant shows phosphoribosylanthranilate isomerase activity Salmonella enterica subsp. enterica serovar Typhimurium
F22C/T24A/K35E/D56N/P62S/E90K/L144I/L177D/I240V the mutant shows phosphoribosylanthranilate isomerase activity Salmonella enterica subsp. enterica serovar Typhimurium
F22C/T24A/K35E/T39A/D56N/G61S/E90K/G98C/L177D/Q210L/I240V the mutant shows phosphoribosylanthranilate isomerase activity Salmonella enterica subsp. enterica serovar Typhimurium
F22Y/T39A/G61S/G98C/D161V/Y203N/A222T the mutant shows phosphoribosylanthranilate isomerase activity Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
r

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium P00929
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelate affinity column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
r

Synonyms

Synonyms Comment Organism
TrpA
-
Salmonella enterica subsp. enterica serovar Typhimurium
tryptophan synthase alpha-subunit
-
Salmonella enterica subsp. enterica serovar Typhimurium