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Literature summary for 4.2.1.20 extracted from

  • Mitsuya, D.; Tanaka, S.; Matsumura, H.; Urano, N.; Takano, K.; Ogasahara, K.; Takehira, M.; Yutani, K.; Ishida, M.
    Strategy for cold adaptation of the tryptophan synthase alpha subunit from the psychrophile Shewanella frigidimarina K14-2: crystal structure and physicochemical properties (2014), J. Biochem., 155, 73-82.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Shewanella frigidimarina

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1M HEPES-Na, pH 7.5, 2% (v/v) polyethylene glycol 400 and 2 M ammonium sulfate, at 4°C Shewanella frigidimarina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate Shewanella frigidimarina
-
L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
?
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate Shewanella frigidimarina K14-2
-
L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Shewanella frigidimarina
-
-
-
Shewanella frigidimarina K14-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
SuperQ-650M column chromatography, Sephacryl S-300HR column chromatography, and MonoQ HR5/5 column chromatography Shewanella frigidimarina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-C-(indol-3-yl)glycerol 3-phosphate
-
Shewanella frigidimarina D-glyceraldehyde 3-phosphate + indole
-
?
1-C-(indol-3-yl)glycerol 3-phosphate
-
Shewanella frigidimarina K14-2 D-glyceraldehyde 3-phosphate + indole
-
?
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate
-
Shewanella frigidimarina L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
?
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate
-
Shewanella frigidimarina K14-2 L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
?

Synonyms

Synonyms Comment Organism
tryptophan synthase alpha subunit
-
Shewanella frigidimarina
TSA
-
Shewanella frigidimarina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42.5 45
-
Shewanella frigidimarina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
the denaturation temperature of the enzyme is 56°C at pH 7.0 Shewanella frigidimarina