Crystallization (Comment) | Organism |
---|---|
crystallization of wild-type enzyme alone and complexed with L-serine and 3-indolylpropanol 3'-phosphate, and of mutant T183V complexed with L-serine, 1-(indol-3-yl)glycerol 3-phosphate, and 3-indolylpropanol 3'-phosphate, hanging drop method in the dark at room temperature, equal volumes of protein solution, containing 10 mg/ml protein, 50 mM Na-bicine, pH 7.8, 10 mM Na-EDTA, 1 mM dithioerythritol, 0.02 mM pyridoxal 5'-phosphate, and of reservoir solution, containing 50 mM Na-bicine, pH 7.8, 5 mM dithioerythritol, 5 mM Na-EDTA, 0.1 mM pyridoxal 5'-phosphate, 2 mM spermine, 8-12% w/v PEG 8000, X-ray diffraction structure determination and analysis at different resolutions 1.45-2.3 A, comparison of crystal structures of the different complexes | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
T183V | site-directed mutagenesis, isosteric alpha-subunit mutant, exchange of a highly conserved residue, strongly impaired allosteric alpha-beta communication, 100fold reduced alpha-reaction activity, beta-reaction activity is not affected, effects are due to a missing hydrogen bind between alphaT183 and the catalytic residue alphaAsp60 | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-glycerol 3-phosphate | inhibits the reaction of indole with the enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), reaction mechanism of alpha- and beta-reaction step, T183 and D60 are involved | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bifunctional enzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
L-serine + indole | beta-subunit of the bifunctional enzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
L-serine + indoline | - |
Salmonella enterica subsp. enterica serovar Typhimurium | dihydro-iso-L-tryptophan + H2O | i.e. DIT | ? | |
additional information | allostery and substrate channeling, wild-type and mutant T183V | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2beta2 complex | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms | Comment | Organism |
---|---|---|
TRPS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
L-serine | overall-reaction, pH 7.8, 25°C, mutant T183V enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.00017 | - |
1-(indol-3-yl)glycerol 3-phosphate | overall-reaction, pH 7.8, 25°C, mutant T183V enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.00059 | - |
1-(indol-3-yl)glycerol 3-phosphate | alpha-reaction, pH 7.8, 25°C, mutant T183V enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.063 | - |
1-(indol-3-yl)glycerol 3-phosphate | alpha-reaction, pH 7.8, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.61 | - |
L-serine | overall-reaction, pH 7.8, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.61 | - |
1-(indol-3-yl)glycerol 3-phosphate | overall-reaction, pH 7.8, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
3 | 6 | L-serine | overall-reaction, pH 7.8, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
3 | 6 | 1-(indol-3-yl)glycerol 3-phosphate | overall-reaction, pH 7.8, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
3.3 | - |
L-serine | beta-reaction, pH 7.8, 25°C, mutant T183V enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
3.3 | - |
indole | beta-reaction, pH 7.8, 25°C, mutant T183V enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
3.3 | - |
L-serine | beta-reaction, pH 7.8, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
3.3 | - |
indole | beta-reaction, pH 7.8, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.51 | - |
L-serine | beta-reaction, pH 7.8, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.51 | - |
indole | beta-reaction, pH 7.8, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
5.24 | - |
L-serine | beta-reaction, pH 7.8, 25°C, mutant T183V enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
5.24 | - |
indole | beta-reaction, pH 7.8, 25°C, mutant T183V enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on, bound to the enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |