Cloned (Comment) | Organism |
---|---|
genes trpA, trpB1, and trpB2, expression in Escherichia coli | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady state kinetics | Thermotoga maritima | |
0.025 | - |
indole | pH 7.5, 80°C, recombinant (TrpA-TrpB1)2 tetrameric complex, beta-reaction | Thermotoga maritima | |
0.04 | - |
indole | pH 7.5, 80°C, recombinant TrpB1, beta-reaction | Thermotoga maritima | |
0.19 | - |
1-(indol-3-yl)glycerol 3-phosphate | pH 7.5, 80°C, recombinant (TrpA-TrpB1)2 tetrameric complex, alpha-reaction | Thermotoga maritima | |
1.62 | - |
1-(indol-3-yl)glycerol 3-phosphate | pH 7.5, 80°C, recombinant TrpA, alpha-reaction | Thermotoga maritima | |
3.7 | - |
L-serine | pH 7.5, 80°C, recombinant (TrpA-TrpB1)2 tetrameric complex, beta-reaction | Thermotoga maritima | |
110 | - |
L-serine | pH 7.5, 80°C, recombinant TrpB1, beta-reaction | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26200 | - |
recombinant TrpA monomer, sedimentation equilibrium | Thermotoga maritima |
91000 | - |
recombinant TrpB1 dimer, sedimentation equilibrium | Thermotoga maritima |
98000 | - |
recombinant TrpB2 dimer, sedimentation equilibrium | Thermotoga maritima |
140000 | - |
recombinant alphabetabetaalpha complex of TRpA and TrpB1, sedimentation equilibrium | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
hyperthermophile, alpha-subunit TrpA and beta-subunit TrpB1 are encoded by the 2 genes trpA and trpB1 in the trp operon, and by a trpB2 gene outside the operon | - |
Purification (Comment) | Organism |
---|---|
recombinant TrpA, TrpB1, and TrpB2 from Escherichia coli | Thermotoga maritima |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Thermotoga maritima | D-glyceraldehyde 3-phosphate + indole | - |
? | |
L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Thermotoga maritima | L-tryptophan + H2O | - |
? | |
additional information | indole is channeled to the active site of the beta-subunit, the TrpB2 homodimer has a high catalytic efficiency due to a low Km for indole, it functions as a rescue protein for indole to prevent the escape of the costly hydrophobic metabolite at the hig growing temperatures of the hyperthermophile | Thermotoga maritima | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | TrpB2 does not bind to the alpha-subunit and forms homodimers, TrpB1 alone forms homodimers | Thermotoga maritima |
monomer | TrpA alone forms monomers | Thermotoga maritima |
More | recombinantly expressed alpha-subunit forms monomers of 26.7 kDa, while the recombinant beta-subunits B1 and B2 both form dimers of 49.4 and 61.7 kDa, respectively | Thermotoga maritima |
tetramer | alphabetabetaalpha complex of TRpA and TrpB1, sedimentation equilibrium | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
TrpA | - |
Thermotoga maritima |
TrpB1 | - |
Thermotoga maritima |
TrpB2 | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | alpha-reaction assay at | Thermotoga maritima |
80 | - |
beta-reaction assay at | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.174 | - |
1-(indol-3-yl)glycerol 3-phosphate | pH 7.5, 80°C, recombinant TrpA, alpha-reaction | Thermotoga maritima | |
3.8 | - |
L-serine | pH 7.5, 80°C, recombinant TrpB1, beta-reaction | Thermotoga maritima | |
4.2 | - |
indole | pH 7.5, 80°C, recombinant TrpB1, beta-reaction | Thermotoga maritima | |
5.6 | - |
1-(indol-3-yl)glycerol 3-phosphate | pH 7.5, 80°C, recombinant (TrpA-TrpB1)2 tetrameric complex, alpha-reaction | Thermotoga maritima | |
8 | - |
L-serine | pH 7.5, 80°C, recombinant (TrpA-TrpB1)2 tetrameric complex, beta-reaction | Thermotoga maritima | |
10 | - |
indole | pH 7.5, 80°C, recombinant (TrpA-TrpB1)2 tetrameric complex, beta-reaction | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermotoga maritima |