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Literature summary for 4.2.1.20 extracted from

  • Marabotti, A.; De Biase, D.; Tramonti, A.; Bettati, S.; Mozzarelli, A.
    Allosteric communication of tryptophan synthase. Functional and regulatory properties of the beta S178P mutant (2001), J. Biol. Chem., 276, 17747-17753.
    View publication on PubMed

Application

Application Comment Organism
analysis the enzyme complex is a model enzyme for understanding allosteric regulation Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

Cloned (Comment) Organism
expressionof mutant S178P in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method, 10 mg/ml purified recombinant mutant S178P enzyme in 50 mM bicine, pH 7.8, with equal volume of reservoir solution: 50 mM bicine, 1 mM EDTA, 12% w/v PEG 8000, 1.4 mM spermine, pH 7.8, 21°C, structure determination and functional investigation by polarized absorption microspectrophotometry Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
S178P site-directed mutagenesis, molecular modeling of the structural effects caused by the mutation, hydrogen bond with alphaGlu181 is interrupted, 2fold decreased activity of the beta-subunit, not affected, in contrast to the wild-type, by allosteric effectors indole-3-acetylglycine and DL-alpha-glycerol-3-phosphate in the beta-reaction, weaker binding of Na+ Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant S178P from Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

Reaction Comment Organism Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium D-glyceraldehyde 3-phosphate + indole
-
?
L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
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?
additional information allosteric communication, Ser178 is a critical residue in ligand-triggered signals between alpha and beta active sites Salmonella enterica subsp. enterica serovar Typhimurium ?
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?

Subunits

Subunits Comment Organism
tetramer alpha2beta2 complex Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
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Salmonella enterica subsp. enterica serovar Typhimurium