Cloned (Comment) | Organism |
---|---|
overexpression of the alpha-subunit in Escherichia coli | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant alpha-subunit, hanging drop vapour diffusion method, 10°C, reservoir solution: 0.1 M MES-NaOH, pH 6.5, 12% PEG 20000, X-ray diffraction structure determination and analysis at 2.0 A resolution | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U094 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant alpha-subunit from Escherichia coli | Pyrococcus furiosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Pyrococcus furiosus | D-glyceraldehyde 3-phosphate + indole | - |
? | |
L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Pyrococcus furiosus | L-tryptophan + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the alpha-subunit of the enzyme has extremely high thermostability, due to increase in ion pairs, decrease in cavity volume, and entropic effects, not by hydrophobic interactions | Pyrococcus furiosus |