Literature summary for 4.2.1.20 extracted from

Mozzarelli, A.; Peracchi, A.; Rovegno, B.; Dale, G.; Rossi, G.L.; Dunn, M.F.
Effect of pH and monovalent cations on the formation of quinonoid intermediates of the tryptophan synthase alpha2beta2 complex in solution and in the crystal (2000), J. Biol. Chem., 275, 6956-6962.

Search for more literature for 4.2.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the enzyme complex in Escherichia coli	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme complex, structure determination and analysis	Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics of formation of quinoid reaction intermediates in the beta-active site upon reaction with the substrates, pH-dependence	Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cs+	affects kinetic properties of the enzyme complex and stabilizes the quinoid intermediates formed	Salmonella enterica subsp. enterica serovar Typhimurium
Na+	affects kinetic properties of the enzyme complex and stabilizes the quinoid intermediates formed	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme complex from Escherichia coli	Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

Reaction	Comment	Organism	Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O	also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), reaction mechanism, formation of quinoid reaction intermediates in the beta-active site upon reaction with the substrates	Salmonella enterica subsp. enterica serovar Typhimurium	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-(indol-3-yl)glycerol 3-phosphate	alpha-subunit of the bienzyme complex, alpha-reaction	Salmonella enterica subsp. enterica serovar Typhimurium	D-glyceraldehyde 3-phosphate + indole	-	?
L-serine + indole	beta-subunit of the bienzyme complex, beta-reaction, detailed determinaion of quinoid reaction intermediates in the beta-active site upon reaction with the substrates	Salmonella enterica subsp. enterica serovar Typhimurium	L-tryptophan + H2O	-	?

Subunits

Subunits	Comment	Organism
tetramer	alpha2beta2 complex	Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)