Literature summary for 4.2.1.20 extracted from

Jeong, M.S.; Jeong, J.K.; Park, K.S.; Kim, H.T.; Lee, K.M.; Lim, W.K.; Jang, S.B.
Crystallization and preliminary X-ray analysis of tryptophan synthase alpha-subunits from Escherichia coli (2004), Acta Crystallogr. Sect. D, 60, 132-134.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of the alpha-subunit in strain RB797	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
10 mg/ml purified recombinant alpha-subunit, hanging drop vapour diffusion method, 298K, equal volume, 0.001 ml, of protein solution and reservoir solution are mixed and placed over 0.5 ml reservoir solution, precipitant solution: 0.5 M ammonium sulfate, 0.1 M trisodium citrate dihydrate, 1.0 M lithium sulfate monohydrate, pH 5.6, first crystals after 7-10 days, maximal size within 2 weeks, X-ray diffraction structure determination and analysis at 2.8 A resolution	Escherichia coli

Crystallization (Comment)

Organism

10 mg/ml purified recombinant alpha-subunit, hanging drop vapour diffusion method, 298K, equal volume, 0.001 ml, of protein solution and reservoir solution are mixed and placed over 0.5 ml reservoir solution, precipitant solution: 0.5 M ammonium sulfate, 0.1 M trisodium citrate dihydrate, 1.0 M lithium sulfate monohydrate, pH 5.6, first crystals after 7-10 days, maximal size within 2 weeks, X-ray diffraction structure determination and analysis at 2.8 A resolution

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant alpha-subunit	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O	also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8)	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-(indol-3-yl)glycerol 3-phosphate	alpha-subunit of the bienzyme complex, alpha-reaction	Escherichia coli	D-glyceraldehyde 3-phosphate + indole	channeling of indole to the beta-subunit active site	?
L-serine + indole	beta-subunit of the bienzyme complex, beta-reaction	Escherichia coli	L-tryptophan + H2O	-	?

Subunits

Subunits	Comment	Organism
More	the alpha-subunit contains no disulfide bond, conformational stabilization mechanism	Escherichia coli
tetramer	alpha2beta2 enzyme complex	Escherichia coli

Synonyms

Synonyms	Comment	Organism
alphaTS	alpha-subunit	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
additional information	the alpha-subunit contains no prosthetic group	Escherichia coli