Literature summary for 4.2.1.20 extracted from

Yang, X.J.; Ruvinov, S.B.; Miles, E.W.
Overexpression and purification of separate tryptophan synthase alpha and beta subunits from Salmonella typhimurium (1992), Protein Expr. Purif., 3, 347-354.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
alpha subunit	Salmonella enterica subsp. enterica serovar Typhimurium
beta subunit	Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability	Organism
-20°C, beta subunit, 50 mM sodium bicine buffer, pH 7.8, 1 mM EDTA, 0.04 mM pyridoxal 5'-phoshate, 2 mM DTT	Salmonella enterica subsp. enterica serovar Typhimurium
-75°C, or liquid N2, alpha subunit	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
indole + L-serine	-	Salmonella enterica subsp. enterica serovar Typhimurium	L-tryptophan + H2O	-	?

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Release 2023.1 (January 2023)