General Stability | Organism |
---|---|
influence of ionic strength, stable in 1 M phosphate buffer, instable in 0.1M or 0.01 M phosphate buffer | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Saccharomyces cerevisiae | |
GSH | - |
Saccharomyces cerevisiae | |
indoleacetic acid | 3.5 mM, 50% inhibition | Saccharomyces cerevisiae | |
indoleacrylic acid | 0.05 mM, 50% inhibition | Saccharomyces cerevisiae | |
indolebutyric acid | 7.5 mM, 50% inhibition | Saccharomyces cerevisiae | |
Indolepropionic acid | 7.5 mM, 50% inhibition | Saccharomyces cerevisiae | |
indolepyruvic acid | 2.3 mM, 50% inhibition | Saccharomyces cerevisiae | |
L-cysteine | - |
Saccharomyces cerevisiae | |
PCMB | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
1-(indol-3-yl)glycerol 3-phosphate | cosubstrate L-Ser | Saccharomyces cerevisiae | |
0.16 | - |
indole | cosubstrate L-Ser | Saccharomyces cerevisiae | |
0.5 | - |
1-(indol-3-yl)glycerol 3-phosphate | no cosubstrate | Saccharomyces cerevisiae | |
5.3 | - |
L-Ser | cosubstrate indole | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
4°C, 1 M potassium phosphate buffer, pH 7.6, 1 mM pyridoxal 5'-phosphate, 2 mM PMSF, 24 h, 7% loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate + L-serine | - |
Saccharomyces cerevisiae | L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
indole + L-serine | - |
Saccharomyces cerevisiae | L-tryptophan + H2O | - |
? | |
indole-3-glycerol phosphate | - |
Saccharomyces cerevisiae | indole + D-glyceraldehyde 3-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7 | - |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
indoleacrylic acid | - |
Saccharomyces cerevisiae | |
2.3 | - |
indolepyruvic acid | - |
Saccharomyces cerevisiae | |
3.5 | - |
indoleacetic acid | - |
Saccharomyces cerevisiae | |
7.5 | - |
Indolepropionic acid | - |
Saccharomyces cerevisiae | |
7.5 | - |
indolebutyric acid | - |
Saccharomyces cerevisiae |