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Literature summary for 4.2.1.20 extracted from

  • Bartholmes, P.; Boeker, H.; Jaenicke, R.
    Purification of tryptophan synthase from Saccharomyces cerevisiae and partial activity of its nicked subunits (1979), Eur. J. Biochem., 102, 167-172.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
indole cosubstrate L-Ser Saccharomyces cerevisiae
0.17
-
indole cosubstrate D-glyceraldehyde 3-phosphate Saccharomyces cerevisiae
1.1
-
D-glyceraldehyde 3-phosphate cosubstrate L-Trp, i.e. reverse reaction Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
2 * 77000, sedimentation equilibrium in 6 M guanidine hydrochloride Saccharomyces cerevisiae
153000
-
sedimentation equilibrium centrifugation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-18°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate + L-serine r Saccharomyces cerevisiae L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
?
indole + D-glyceraldehyde 3-phosphate r Saccharomyces cerevisiae indole-3-glycerol phosphate r ?
indole + L-serine
-
Saccharomyces cerevisiae L-tryptophan + H2O
-
?
indole-3-glycerol phosphate r Saccharomyces cerevisiae indole + D-glyceraldehyde 3-phosphate r ?
L-tryptophan + D-glyceraldehyde 3-phosphate + H2O r Saccharomyces cerevisiae indole-3-glycerol phosphate + L-serine r ?

Subunits

Subunits Comment Organism
dimer 2 * 77000, sedimentation equilibrium in 6 M guanidine hydrochloride Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km: 0.18 mM Saccharomyces cerevisiae