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Literature summary for 4.2.1.2 extracted from

  • van Vugt-Lussenburg, B.M.; van der Weel, L.; Hagen, W.R.; Hagedoorn, P.L.
    Biochemical similarities and differences between the catalytic [4Fe-4S] cluster containing fumarases FumA and FumB from Escherichia coli (2013), PLoS ONE, 8, e55549.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

General Stability

General Stability Organism
exposure to air results in 30% decreased activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
L-malate pH 8, 37°C, Vmax: 490 micromol/minute/mg/ Escherichia coli
0.32
-
fumarate pH 8, 37°C, Vmax: 1430 micromol/minute/mg/ Escherichia coli
0.46
-
fumarate pH 8, 37°C, Vmax: 1900 micromol/minute/mg/ Escherichia coli
0.7
-
D-Tartrate pH 8, 37°C, Vmax: 2.3 micromol/minute/mg/ Escherichia coli
0.7
-
L-malate pH 8, 37°C, Vmax: 720 micromol/minute/mg/ Escherichia coli
0.8
-
D-Tartrate pH 8, 37°C, Vmax: 9.2 micromol/minute/mg/ Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
[3Fe-4S] center 10-25% of purified protein contains [3Fe-4S] cluster Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-tartrate
-
Escherichia coli ?
-
?
L-malate
-
Escherichia coli fumarate + H2O
-
r

Subunits

Subunits Comment Organism
homodimer
-
Escherichia coli

Synonyms

Synonyms Comment Organism
FumA
-
Escherichia coli
FumB
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
D-Tartrate pH 8, 37°C Escherichia coli
12
-
D-Tartrate pH 8, 37°C Escherichia coli
1000
-
L-malate pH 8, 37°C/ Escherichia coli
1600
-
L-malate pH 8, 37°C Escherichia coli
4200
-
fumarate pH 8, 37°C Escherichia coli
4500
-
fumarate pH 8, 37°C Escherichia coli