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Literature summary for 4.2.1.2 extracted from

  • Su, R.R.; Wang, A.; Hou, S.T.; Gao, P.; Zhu, G.P.; Wang, W.
    Identification of a novel fumarase C from Streptomyces lividans TK54 as a good candidate for L-malate production (2014), Mol. Biol. Rep., 41, 497-504.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate
-
Streptomyces lividans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Streptomyces lividans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.7
-
L-malate pH 6.5, 40°C Streptomyces lividans
15
-
L-malate pH 7.3, 40°C Streptomyces lividans
57
-
L-malate pH 8, 40°C Streptomyces lividans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
SDS-PAGE Streptomyces lividans

Organism

Organism UniProt Comment Textmining
Streptomyces lividans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + H2O
-
Streptomyces lividans L-malate
-
?

Synonyms

Synonyms Comment Organism
fumarase C
-
Streptomyces lividans
slFumC
-
Streptomyces lividans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Streptomyces lividans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
over 85% of activity after 48 h incubation at 40°C Streptomyces lividans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
65
-
L-malate pH 6.5, 40°C Streptomyces lividans
267
-
L-malate pH 7.3, 40°C Streptomyces lividans
620
-
L-malate pH 8, 40°C Streptomyces lividans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Streptomyces lividans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.7
-
L-malate pH 6.5, 40°C Streptomyces lividans
10.88
-
L-malate pH 8, 40°C Streptomyces lividans
17.8
-
L-malate pH 7.3, 40°C Streptomyces lividans