Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as His-tagged fusion proteins | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
S-malate | pH 8.7, temperature not specified in the publication, aerobic condition, Vmax: 0.5 micromol/min/mg | Leishmania major | |
1.3 | - |
fumarate | pH 8.7, temperature not specified in the publication, aerobic condition, Vmax: 1.8 micromol/min/mg | Leishmania major | |
1.9 | - |
fumarate | pH 9, temperature not specified in the publication, aerobic condition, Vmax: 44.3 micromol/min/mg | Leishmania major | |
2.3 | - |
S-malate | pH 8.7, temperature not specified in the publication, anaerobic condition, Vmax: 11.8 micromol/min/mg | Leishmania major | |
2.5 | - |
fumarate | pH 8.7, temperature not specified in the publication, anaerobic condition, Vmax: 26.4 micromol/min/mg | Leishmania major | |
5.7 | - |
S-malate | pH 9, temperature not specified in the publication, aerobic condition, Vmax: 22.7 micromol/min/mg | Leishmania major | |
5.7 | - |
fumarate | pH 9, temperature not specified in the publication, anaerobic condition, Vmax: 186 micromol/min/mg | Leishmania major | |
12.6 | - |
S-malate | pH 9, temperature not specified in the publication, anaerobic condition, Vmax: 138 micromol/min/mg | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leishmania major | 5829 | - |
glycosome | - |
Leishmania major | 20015 | - |
mitochondrion | - |
Leishmania major | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61100 | - |
calculated from cDNA | Leishmania major |
62600 | - |
calculated from cDNA | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-malate | - |
Leishmania major | fumarate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LmFH-1 | - |
Leishmania major |
LmFH-2 | - |
Leishmania major |
LmjF24.0320 | - |
Leishmania major |
LmjF29.1960 | - |
Leishmania major |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56.3 | - |
melting temperature | Leishmania major |
57.7 | - |
melting temperature | Leishmania major |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
S-malate | pH 8.7, temperature not specified in the publication, aerobic condition | Leishmania major | |
1.9 | - |
fumarate | pH 8.7, temperature not specified in the publication, aerobic condition | Leishmania major | |
12.9 | - |
S-malate | pH 8.7, temperature not specified in the publication, anaerobic condition | Leishmania major | |
24.9 | - |
S-malate | pH 9, temperature not specified in the publication, aerobic condition | Leishmania major | |
28.3 | - |
fumarate | pH 8.7, temperature not specified in the publication, anaerobic condition | Leishmania major | |
48.6 | - |
fumarate | pH 9, temperature not specified in the publication, aerobic condition | Leishmania major | |
151.4 | - |
S-malate | pH 9, temperature not specified in the publication, anaerobic condition | Leishmania major | |
204.2 | - |
fumarate | pH 9, temperature not specified in the publication, anaerobic condition | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Leishmania major |
9 | - |
- |
Leishmania major |