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Literature summary for 4.2.1.2 extracted from

  • Jiang, C.; Wu, L.L.; Zhao, G.C.; Shen, P.H.; Jin, K.; Hao, Z.Y.; Li, S.X.; Ma, G.F.; Luo, F.F.; Hu, G.Q.; Kang, W.L.; Qin, X.M.; Bi, Y.L.; Tang, X.L.; Wu, B.
    Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms (2010), Microb. Cell Fact., 9, 91.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.2

Application

Application Comment Organism
synthesis fumarase is used for the industrial production of L-malate from the substrate fumarate uncultured marine microorganism

Cloned(Commentary)

Cloned (Comment) Organism
gene fumF, cloned from a plasmid metagenomic library, DNA and amino acid sequence determination and analysis, sequence comparison, and phylogenetic analysis uncultured marine microorganism

Inhibitors

Inhibitors Comment Organism Structure
CuCl2 inhibits 37% at 5 mM uncultured marine microorganism
EDTA inhibits 20% at 2.5 mM uncultured marine microorganism
FeCl2 inhibits 37% at 5 mM uncultured marine microorganism
FeCl3 inhibits 47.3% at 5 mM uncultured marine microorganism
NH4Cl inhibits 23% at 5 mM uncultured marine microorganism
SDS
-
 uncultured marine microorganism
ZnCl2 inhibits 55% at 5 mM uncultured marine microorganism

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, stimulates 10% at 5 mM uncultured marine microorganism
additional information class II fumarase activity is frequently influenced by divalent metal ions and non-ionic surfactants. No effect by Ca2+ uncultured marine microorganism

Organism

Organism UniProt Comment Textmining
uncultured marine microorganism
-
 gene fumF
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
726
-
 purified recombinant FumF, pH 8.5, 55°C, substrate fumarate uncultured marine microorganism

Synonyms

Synonyms Comment Organism
class II fumarase
-
 uncultured marine microorganism
fumarase
-
 uncultured marine microorganism
FumF
-
 uncultured marine microorganism

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 uncultured marine microorganism

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 65 70% of maximum activity below 40°C, and over 70% of maximum activity between 40-60°C, maximal activity at approximately 55°C uncultured marine microorganism

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 70 30 min, pH 8.5, activity of FumF is stable with increasing temperature below 58°C in the absence of any stabilizer. When the temperature is higher than 60°C, FumF protein loses almost 50% of its activity uncultured marine microorganism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 uncultured marine microorganism

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH optimum at pH 8.5, the activity is dramatically suppressed below pH 7.0 and above pH 9.0 uncultured marine microorganism

General Information

General Information Comment Organism
metabolism fumarase catalyzes the reversible hydration of fumarate to L-malate and is a key enzyme in the tricarboxylic acid cycle and in amino acid metabolism uncultured marine microorganism