Literature summary for 4.2.1.2 extracted from

Ueda, Y.; Yumoto, N.; Tokushige, M.; Fukui, K.; Ohya-Nishiguchi, H.
Purification and characterization of two types of fumarase from Escherichia coli (1991), J. Biochem., 109, 728-733.

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General Stability

General Stability	Organism
(NH4)2SO4, protects enzyme form FUMA from inactivation at 4°C	Escherichia coli
10% glycerol, 0.1 M KCl, 5 mM L-malate or 30% ethyleneglycol, partially protects enzyme form FUMA from inactivation at 4°C	Escherichia coli
enzyme form FUMA, oxidation and the concomitant release of iron inactivates the enzyme in a reversible manner	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
ammonium persulfate	-	Escherichia coli
pyromellilate	-	Escherichia coli
S-2,3-dicarboxyaziridine	enzyme form FUMC is inhibited, enzyme form FUMA is not inhibited	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.049	-	L-malate	enzyme form FUMA	Escherichia coli
0.05	-	L-malate	enzyme form FUMC	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	one Fe-S-dependent enzyme form and one Fe-S-independent enzyme form	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	2 * 60000, enzyme form FUMA, SDS-PAGE	Escherichia coli
120000	-	enzyme form FUMA, gel filtration	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	2 types of enzyme: Fe-independent FUMC and Fe-dependent FUMA	-
Escherichia coli	-	W	-

Purification (Commentary)

Purification (Comment)	Organism
FUMA and FUMC	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
340	-	FUMA	Escherichia coli
730	-	FUMC	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-malate	-	Escherichia coli	fumarate + H2O	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 60000, enzyme form FUMA, SDS-PAGE	Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
49	-	5 min, 50% inhibition, enzyme form FUMA	Escherichia coli
51	-	5 min, 50% inhibition, enzyme form FUMA	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	enzyme form FUMC	Escherichia coli
8.5	-	enzyme form FUMA	Escherichia coli

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Release 2023.1 (January 2023)