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Literature summary for 4.2.1.2 extracted from
- Characterization of multiple fumarase proteins in Escherichia coli (1988), Biochem. Biophys. Res. Commun., 153, 1236-1243.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | one Fe-S-dependent enzyme form and one Fe-S-independent enzyme form | Escherichia coli |  |
| Iron | in cells grown without aeration the Fe-S-independent enzyme form occupies over 80% of the overall fumarase. In aerobically grown cells the Fe-S-dependent fumarase occupies 80% of the overall activity | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
4 * 48000, Fe-S-independent enzyme form, SDS-PAGE | Escherichia coli |
| 200000 | - |
Fe-S-independent enzyme form, gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
one Fe-S-dependent enzyme form and one Fe-S-independent enzyme form | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, activity readily decreases to less than 20% | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate | - |
Escherichia coli | fumarate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 48000, Fe-S-independent enzyme form, SDS-PAGE | Escherichia coli |
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