Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 trans-2-decenoyl-CoA + 2 H2O | Pseudomonas aeruginosa enzyme activity is of both the ECH-1 and ECH-2 type | Pseudomonas aeruginosa | (3S)-3-hydroxydecanoyl-CoA + (3R)-3-hydroxydecanoyl-CoA | R- and S-enantiomers of produced 3-hydroxydecanoate are nearly equally abundant in case of Pseudomonas aeruginosa | ? | |
additional information | development of a chiral high-performance liquid chromatography-tandem mass spectrometry method for analysis of stereospecificity of enoyl-coenzyme A hydratases/isomerases, including reaction of the 3-hydroxyl group on the chiral carbon with 3,5-dimethylphenyl isocyanate, resolving of the resulting urethane derivatives, and monitoring of the liberated free hydroxy fatty acid fragment ion, detailed overview | Escherichia coli | ? | - |
? | |
additional information | development of a chiral high-performance liquid chromatography-tandem mass spectrometry method for analysis of stereospecificity of enoyl-coenzyme A hydratases/isomerases, including reaction of the 3-hydroxyl group on the chiral carbon with 3,5-dimethylphenyl isocyanate, resolving of the resulting urethane derivatives, and monitoring of the liberated free hydroxy fatty acid fragment ion, detailed overview | Pseudomonas aeruginosa | ? | - |
? | |
trans-2-decenoyl-CoA + H2O | Escherichia coli enzyme activity is of the S-specific ECH-1 type | Escherichia coli | (3S)-3-hydroxydecanoyl-CoA | the distribution of R- and S-enantiomers of produced 3-hydroxydecanoate is in favour of the S-enantiomer in case of Escherichia coli | ? | |
trans-2-decenoyl-CoA + H2O | Escherichia coli enzyme activity is of the S-specific ECH-1 type | Escherichia coli | (3R)-3-hydroxydecanoyl-CoA | the distribution of R- and S-enantiomers of produced 3-hydroxydecanoate is in favour of the S-enantiomer in case of Escherichia coli | ? |
Synonyms | Comment | Organism |
---|---|---|
ECH-1 | S-specific | Escherichia coli |
ECH-1 | S-specific | Pseudomonas aeruginosa |
ECH-2 | R-specific | Escherichia coli |
ECH-2 | R-specific | Pseudomonas aeruginosa |
R-3-hydroxyacyl-CoA enoyl-CoA hydratase | - |
Pseudomonas aeruginosa |
R-3-hydroxyacyl-CoA enoyl-CoA hydratases | - |
Escherichia coli |
S-3-hydroxyacyl-CoA enoyl-CoA hydratase | - |
Pseudomonas aeruginosa |
S-3-hydroxyacyl-CoA enoyl-CoA hydratases | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
8 | - |
assay at | Pseudomonas aeruginosa |