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Literature summary for 4.2.1.17 extracted from

  • Kasaragod, P.; Venkatesan, R.; Kiema, T.R.; Hiltunen, J.K.; Wierenga, R.K.
    The crystal structure of liganded rat peroxisomal multifunctional enzyme type 1: a flexible molecule with two interconnected active sites (2010), J. Biol. Chem., 285, 24089-24098.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichis coli BL21(DE3) Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
at 2.8 A resolution, multidomain protein having 5 domains: A, B, C, D, and E. The N-terminal part has a crotonase fold, which builds the active site for the DELTA3,DELTA2-enoyl-CoA isomerase (EC 5.3.3.8) and DELTA2-enoyl-CoA hydratase-1 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Rattus norvegicus 5777
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P07896
-
-

Purification (Commentary)

Purification (Comment) Organism
purified from rat liver Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-enoyl-CoA + H2O 2E-enoyl-CoA is the product of the DELTA3,DELTA2-enoyl-CoA isomerase (EC 5.3.3.8) reaction, which subsequently is converted into (3S)-hydroxyacyl-CoA in the hydration step Rattus norvegicus (3S)-hydroxyacyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
DELTA2-enoyl-CoA hydratase-1
-
 Rattus norvegicus
rat peroxisomal multifunctional enzyme type 1
-
 Rattus norvegicus
rpMFE1
-
 Rattus norvegicus

General Information

General Information Comment Organism
physiological function the multifunctional enzyme is involved in an alpha-methylacyl-CoAracemase-MFE2 independent synthesis pathway of bile acids from (24S)-hydroxyoxisterols, is involved in the beta-oxidation of long chain dicarboxylic acids Rattus norvegicus