Crystallization (Comment) | Organism |
---|---|
all variants crystallized in the orthorhombic P2(1) 2(1) 2 (1) space group and are highly isomorphous with approximate unit cell dimensions a=42, b=72 and c=75 A and diffract between 1.56 and 2.0 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L100H/L224S/L240P | kcat/Km (CO2) decreased compared to wild-type, T(M): 65°C compared to wild-type: 58°C, T (inactivation): 65°C compared to wild-type: 60°C | Homo sapiens |
L100H/L224S/L240P/Y7F | kcat/Km (CO2) slightly decreased compared to wild-type, T(M): 61.1°C compared to wild-type: 58°C, T (inactivation): 65°C compared to wild-type: 60°C | Homo sapiens |
L100H/L224S/L240P/Y7F/N62L | kcat/Km (CO2) decreased compared to wild-type, T(M): 58.1°C compared to wild-type: 58°C, T (inactivation): 60°C compared to wild-type: 60°C | Homo sapiens |
L100H/L224S/L240P/Y7F/N62L/N67Q | kcat/Km (CO2) slightly decreased compared to wild-type, T(M): 59.2°C compared to wild-type: 58°C, T (inactivation): 60°C compared to wild-type: 60°C | Homo sapiens |
L100H/L224S/L240P/Y7F/N67Q | kcat/Km (CO2) decreased compared to wild-type, T(M): 62.7°C compared to wild-type: 58°C, T (inactivation): 65°C compared to wild-type: 60°C | Homo sapiens |
Y7F | kcat/Km (CO2) equal to wild-type, T(M): 53°C compared to wild-type: 58°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00918 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Homo sapiens | H2CO3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hCAII | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
T(M), wild-type | Homo sapiens |
60 | - |
inactivation temperature wild-type | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
85000 | - |
CO2 | mutant L100H/L224S/L240P, pH 7.6, 25°C | Homo sapiens | |
88000 | - |
CO2 | mutant L100H/L224S/L240P/Y7F/N62L, pH 7.6, 25°C | Homo sapiens | |
94000 | - |
CO2 | mutant L100H/L224S/L240P/Y7F/N67Q, pH 7.6, 25°C | Homo sapiens | |
110000 | - |
CO2 | mutant L100H/L224S/L240P/Y7F, pH 7.6, 25°C | Homo sapiens | |
110000 | - |
CO2 | mutant L100H/L224S/L240P/Y7F/N62L/N67Q, pH 7.6, 25°C | Homo sapiens | |
120000 | - |
CO2 | mutant Y7F, pH 7.6, 25°C | Homo sapiens | |
120000 | - |
CO2 | wild-type, pH 7.6, 25°C | Homo sapiens |