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Literature summary for 4.2.1.1 extracted from

  • Del Prete, S.; Isik, S.; Vullo, D.; De Luca, V.; Carginale, V.; Scozzafava, A.; Supuran, C.T.; Capasso, C.
    DNA cloning, characterization, and inhibition studies of an alpha-carbonic anhydrase from the pathogenic bacterium Vibrio cholerae (2012), J. Med. Chem., 55, 10742-10748.
    View publication on PubMed
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli as a His-tagged-fusion protein Vibrio cholerae serotype O1

Inhibitors

Inhibitors Comment Organism Structure
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
-
 Vibrio cholerae serotype O1
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
-
 Vibrio cholerae serotype O1
1,2-benzothiazol-3(2H)-one 1,1-dioxide
-
 Vibrio cholerae serotype O1
1-(1,2-benzoxazol-3-yl)methanesulfonamide
-
 Vibrio cholerae serotype O1
2-aminobenzenesulfonamide
-
 Vibrio cholerae serotype O1
2-hydrazinylbenzenesulfonamide
-
 Vibrio cholerae serotype O1
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide
-
 Vibrio cholerae serotype O1
4,5-dichlorobenzene-1,3-disulfonamide
-
 Vibrio cholerae serotype O1
4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide
-
 Vibrio cholerae serotype O1
4-(2-aminoethyl)benzenesulfonamide
-
 Vibrio cholerae serotype O1
4-(2-hydroxyethyl)benzenesulfonamide
-
 Vibrio cholerae serotype O1
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide
-
 Vibrio cholerae serotype O1
4-(aminomethyl)benzenesulfonamide
-
 Vibrio cholerae serotype O1
4-(hydroxymethyl)benzenesulfonamide
-
 Vibrio cholerae serotype O1
4-amino-3-bromobenzenesulfonamide
-
 Vibrio cholerae serotype O1
4-amino-3-chlorobenzenesulfonamide
-
 Vibrio cholerae serotype O1
4-amino-3-fluorobenzenesulfonamide
-
 Vibrio cholerae serotype O1
4-amino-3-iodobenzenesulfonamide
-
 Vibrio cholerae serotype O1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
 Vibrio cholerae serotype O1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
 Vibrio cholerae serotype O1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
 Vibrio cholerae serotype O1
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide
-
 Vibrio cholerae serotype O1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
 Vibrio cholerae serotype O1
4-Aminobenzenesulfonamide
-
 Vibrio cholerae serotype O1
4-hydrazinylbenzenesulfonamide
-
 Vibrio cholerae serotype O1
4-sulfamoylbenzoic acid
-
 Vibrio cholerae serotype O1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
 Vibrio cholerae serotype O1
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide
-
 Vibrio cholerae serotype O1
5-(3-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
 Vibrio cholerae serotype O1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
 Vibrio cholerae serotype O1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
 Vibrio cholerae serotype O1
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide
-
 Vibrio cholerae serotype O1
6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide
-
 Vibrio cholerae serotype O1
6-ethoxy-1,3-benzothiazole-2-sulfonamide
-
 Vibrio cholerae serotype O1
6-hydroxy-1,3-benzothiazole-2-sulfonamide
-
 Vibrio cholerae serotype O1
benzenesulfonamide
-
 Vibrio cholerae serotype O1
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide
-
 Vibrio cholerae serotype O1
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide
-
 Vibrio cholerae serotype O1
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
-
 Vibrio cholerae serotype O1
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate
-
 Vibrio cholerae serotype O1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.7
-
 CO2 pH 7.5, 20°C Vibrio cholerae serotype O1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 SDS-PAGE Vibrio cholerae serotype O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
 Vibrio cholerae serotype O1 H2CO3
-
 ?

Synonyms

Synonyms Comment Organism
alpha-carbonic anhydrase
-
 Vibrio cholerae serotype O1
VchCA
-
 Vibrio cholerae serotype O1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
823000
-
 CO2 pH 7.5, 20°C Vibrio cholerae serotype O1

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000059
-
 5-(3-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.00000069
-
 6-ethoxy-1,3-benzothiazole-2-sulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000025
-
 (4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000036
-
 N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000042
-
 5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000042
-
 4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000047
-
 6-hydroxy-1,3-benzothiazole-2-sulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000063
-
 (4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000068
-
 N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.000008
-
 N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000121
-
 5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000235
-
 5-amino-1,3,4-thiadiazole-2-sulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000303
-
 4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000371
-
 4,5-dichlorobenzene-1,3-disulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000427
-
 4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000453
-
 4-amino-6-chlorobenzene-1,3-disulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000521
-
 2-hydrazinylbenzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000545
-
 4-(hydroxymethyl)benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000567
-
 4-(2-hydroxyethyl)benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000598
-
 4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000629
-
 4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000715
-
 4-sulfamoylbenzoic acid pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000795
-
 6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000884
-
 4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000897
-
 4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.0000991
-
 4-amino-3-iodobenzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.000125
-
 4-hydrazinylbenzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.000133
-
 4-amino-3-bromobenzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.000139
-
 4-amino-3-chlorobenzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.000199
-
 4-amino-3-fluorobenzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.000219
-
 benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.000402
-
 4-(2-aminoethyl)benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.00044
-
 2-aminobenzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.000447
-
 4-(aminomethyl)benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.000471
-
 4-Aminobenzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.000982
-
 1-(1,2-benzoxazol-3-yl)methanesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.001
-
 4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide pH 7.5, 20°C Vibrio cholerae serotype O1
0.001
-
 1,2-benzothiazol-3(2H)-one 1,1-dioxide pH 7.5, 20°C Vibrio cholerae serotype O1
0.001
-
 [(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate pH 7.5, 20°C Vibrio cholerae serotype O1
0.001
-
 2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide pH 7.5, 20°C Vibrio cholerae serotype O1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
70000
-
 CO2 pH 7.5, 20°C Vibrio cholerae serotype O1