Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.1 extracted from

  • Kumar, R.S.; Hendrick, W.; Correll, J.B.; Patterson, A.D.; Melville, S.B.; Ferry, J.G.
    Biochemistry and physiology of the beta class carbonic anhydrase (Cpb) from Clostridium perfringens strain 13 (2013), J. Bacteriol., 195, 2262-2269.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1
-
CO2 pH not specified in the publication, 25°C Clostridium perfringens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ enzyme contains one active site zinc per subunit Clostridium perfringens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
SDS-PAGE Clostridium perfringens
88000
-
gel filtration, tetramer Clostridium perfringens

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Clostridium perfringens H2CO3
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 23000, gel filtration Clostridium perfringens

Synonyms

Synonyms Comment Organism
CPB
-
Clostridium perfringens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Clostridium perfringens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15000
-
CO2 pH not specified in the publication, 25°C Clostridium perfringens

General Information

General Information Comment Organism
malfunction analyses of a cpb deletion mutant shows that Cpb is strictly required for growth when cultured in semi-defined medium and an atmosphere without CO2. Growth of the mutant is the same as that of the parent wild-type strain when cultured in nutrient-rich media with or without CO2 in the atmosphere, although elimination of glucose results in decreased production of acetate, propionate, and butyrate Clostridium perfringens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4800
-
CO2 pH not specified in the publication, 25°C Clostridium perfringens