Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | competitive, strong inhibition of carbonic anhydrase esterase activity | Dicentrarchus labrax | |
Al3+ | competitive, very strong inhibition of carbonic anhydrase esterase activity | Dicentrarchus labrax | |
Co3+ | competitive, strong inhibition of carbonic anhydrase esterase activity | Dicentrarchus labrax | |
Cu2+ | competitive, very strong inhibition of carbonic anhydrase esterase activity | Dicentrarchus labrax | |
Hg2+ | competitive, strong inhibition of carbonic anhydrase esterase activity | Dicentrarchus labrax | |
additional information | the inhibitory metals might be dangerous at low micromolar concentrations for fish carbonic anhydrase enzymes | Dicentrarchus labrax | |
Pb2+ | competitive, moderate inhibition of carbonic anhydrase esterase activity | Dicentrarchus labrax | |
Zn2+ | competitive, strong inhibition of carbonic anhydrase esterase activity | Dicentrarchus labrax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of the esterase activity with 4-nitrophenylacetate as substrate | Dicentrarchus labrax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme shows an optimum ionic strength at 10mM | Dicentrarchus labrax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30200 | - |
x * 30200, SDS-PAGE | Dicentrarchus labrax |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dicentrarchus labrax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 78.8fold from liver by single step tyrosine sulfanilamide affinity chromatography | Dicentrarchus labrax |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Dicentrarchus labrax | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
751.7 | - |
purified enzyme, esterase activity with 4-nitrophenylacetate as substrate, pH 7.5, 25°C | Dicentrarchus labrax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also shows esterase activity with 4-nitrophenylacetate as substrate | Dicentrarchus labrax | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30200, SDS-PAGE | Dicentrarchus labrax |
Synonyms | Comment | Organism |
---|---|---|
carbonic anhydrase | - |
Dicentrarchus labrax |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
optimum temperature for the esterase activity with 4-nitrophenylacetate as substrate | Dicentrarchus labrax |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 70 | assay range | Dicentrarchus labrax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
optimum pH for the esterase activity with 4-nitrophenylacetate as substrate | Dicentrarchus labrax |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
purified native enzyme, optimally stable, 93% of maximum activity remaining after 14 days in 10 mM Tris-HCl buffer, pH 8.5 | Dicentrarchus labrax |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki values of esterase activity with 4-nitrophenylacetate as substrate | Dicentrarchus labrax |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
IC50 values of esterase activity with 4-nitrophenylacetate as substrate, competitive inhibition type | Dicentrarchus labrax | additional information |