Crystallization (Comment) | Organism |
---|---|
carbonic anhydrase II complexed with two aromatic sulfonamide inhibitors N-(4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide and N-(2-fluoro-4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide incorporating 2-thienylacetamido moieties, hanging drop vapor diffusion method, drops of 0.005 ml containing 0.5 mM hCA II, 1 mM inhibitor, 0.1% DMSO, 0.8 M sodium citrate, 50 mM Tris-HCl, pH 8.0,are equilibrated against 1 ml precipitant solution containing 1.6 M sodium citrate and 50 mM Tris-HCl, pH 8.0, at room temperature, X-ray diffraction structure determination and analysis at 1.6-1.7 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetazolamide | - |
Homo sapiens | |
dichlorophenamide | - |
Homo sapiens | |
methazolamide | - |
Homo sapiens | |
additional information | an additional 3-fluoro substituent on the 4-amino-benzenesulfonamide scaffold of inhibitors alters the inhibition profiles against the cytosolic isozymes hCA I, II, III, VII and XIII, interaction analysis, overview. The binding differences render the inhibitors more isozyme-specific. Docking studies, overview | Homo sapiens | |
N-(2-fluoro-4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | - |
Homo sapiens | |
N-(4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic isozymes hCA I, II, III, VII and XIII | Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes hCA I, II, III, VII and XIII | - |
Synonyms | Comment | Organism |
---|---|---|
carbonic anhydrase | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000062 | - |
N-(4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | inhibition of CO2 hydration by isozyme hCAVII, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.000007 | - |
N-(2-fluoro-4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | inhibition of CO2 hydration by isozyme hCAVII, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.00005 | - |
N-(4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | inhibition of CO2 hydration by isozyme hCAII, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.000061 | - |
N-(4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | inhibition of CO2 hydration by isozyme hCAI, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.000125 | - |
N-(4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | inhibition of CO2 hydration by isozyme hCAXIII, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.000161 | - |
N-(2-fluoro-4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | inhibition of CO2 hydration by isozyme hCAI, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.00039 | - |
N-(2-fluoro-4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | inhibition of CO2 hydration by isozyme hCAII, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.000437 | - |
N-(2-fluoro-4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | inhibition of CO2 hydration by isozyme hCAXIII, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.08 | - |
N-(4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | inhibition of CO2 hydration by isozyme hCAIII, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.12 | - |
N-(2-fluoro-4-sulfamoylphenyl)-2-(thiophen-2-yl)acetamide | inhibition of CO2 hydration by isozyme hCAIII, pH 7.5, temperature not specified in the publication | Homo sapiens |